These free H+ ions will protonate any free acetate ions in solutions thus favoring the product of this equilibrium reaction. If we lower the pH of a solution by adding a strong acid like HCl, there will be an abundance of H+ in the solution. Disregard the complex ICE charts for calculating dissociation instead, look at what drives this reaction towards the right or left. If you find your acid/base knowledge weak, start by reviewing my acid/base video series, reading this (link) acid/base overview article, and downloading my free acid/base cheat sheet.Ī carboxylic acid in solution will exist in equilibrium with carboxylate or acetate, its conjugate base. Let’s compare the carboxyl and amine groups to simple molecules like acetic acid and methyl amine. (Learn more about amino acid side chains link) Finding Charge on a Carboxyl Group Acidic and basic amino acids may have additional groups in their side chains. The standard structure contains both a carboxyl and an amine in the backbone. This is the zwitterion form of an amino acid While neutral, the zwitterion form of an amino acid will have a positive and a negative charge. Zwitterion comes from the German word for ‘two’ (zwei) or ‘double’ (zwitter) and the word ‘ion’. That’s not because they have no charge: it’s because their charges balance or cancel out. This drawing is simplified, and is actually incorrect.Īt physiological pH, amino acids will exist with a net charge of zero. Looking at this standard drawing of an amino acid, it appears to be neutral–how does that relate to the pl? The isoelectric point or pI of an amino acid is the pH at which an amino acid has a net charge of zero. The word isoelectric or isoelectronic comes from ‘iso,’ which means the same, and ‘electric,’ which implies charge. When you think of amino acid charge, the first related topic that comes to mind is isoelectric point. While the math concept may be tricky at first, you’ll find that once it makes sense, it’s fairly easy to apply these critical skills for the MCAT. The MCAT is a test of logic and understanding, which is why this article will break down amino acid charge and calculations from a logical application perspective. Understanding why an amino acid will gain or lose a proton at a given pH is even more critical. Knowing how to find both charge and isoelectric point for amino acids is a critical MCAT skill.
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